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KMID : 0364820090450020140
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Korean Journal of Microbiology
2009 Volume.45 No. 2 p.140 ~ p.147
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Analysis of Microbial Community During the Anaerobic Dechlorination of Tetrachloroethylene(PCE) in Stream of Gimpo and Inchon Areas
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Kim Byung-Hyuk
Cho Dae-Hyun
Sung Youl-Boong
Ahn Chi-Yong
Oh Hee-Mock
Koh Sung-Cheol
Kim Hee-Sik
Baek Kyung-Hwa
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Abstract
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In this study, anaerobic enrichment cultivation was performed with the sediments from the Gimpo and Inchon areas. Lactate as an electron donor and PCE as an electron acceptor was injected into the serum bottle with an anaerobic medium. After the incubation of 8 weeks, the reductive dechlorination of PCE was observed in 7 sites among 16 sites (43%). Three enrichment cultures showed completely dechlorination of PCE to ethene, while four enrichment culture showed transformation of PCE to cis-DCE. The bacterial community structure was analyzed by PCR-DGGE. Dechlorinating bacteria were detected by species-specific primers. The dominant species in seven anaerobic enrichments were found to belong to the genus of Dehalococcoides sp. and Geobacter sp., and Dehalobacter sp.
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KEYWORD
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Dehalococcoides sp., Geobacter sp., PCR-DGGE, reductive dechlorination
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